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Fluorescence and Fluorescent dyes - Coggle Diagram
Fluorescence and Fluorescent dyes
What factors affect it
Nature of the Molecule:
Not all molecules fluoresce
The molecular structure, plays a role in determining if a molecule is fluorescent.
Excitation Wavelength:
Each molecule has an optimal excitation wavelength that produces the maximum fluorescence emission.
The intensity of fluorescence can depend on the wavelength of the exciting light.
Quantum Yield:
Refers to the efficiency of the fluorescence process.
It's the ratio of the number of photons emitted to the number of photons absorbed.
Environmental Factors:
PH
The acidity or basicity of the environment can affect the protonation state of the molecule, influencing its fluorescence.
Temperature
Higher temperatures generally reduce fluorescence due to increased non-radiative processes.
Viscosity
In more viscous environments, molecular rotation and vibration might be restricted, which can influence fluorescence.
Solvent
The polarity and nature of the solvent can affect fluorescence. Some solvents can enhance while others can quench fluorescence.
What applications it has on natural sciences
Chemistry
Analyze and detect minerals and metal elements
Medicine
Distinguish between various bacterial species and also enable to classify the bacteria into genus, species and groups.
What is flluorecence?
It is a form of luminescence.
The emission of light by a substance that has absorbed light or other electromagnetic radiation
Emitted light has a longer wavelength
How fluorescence works
Some molecules are capable of being excited, via absorption of light energy, to a higher energy state, also called an excited state
The energy of the excited state—which cannot be sustained for long— “decays” or decreases, resulting in the emission of light energy