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Oral Exfoliative Cytology: A Diagnostic Method in Dentistry, daniela rios…

- - - - Suction point
        
        When there is a closed cavity and we want to collect the cells that have been flaked inside its lumen, we can puncture with a syringe and after aspirating the contents deposit it on a slide for expansion and fixation
      - scraping
        
        Obtaining Cellular Material in Clinically Suspicious Areas
      - Crop or energy rinse
        
        Sample collection method limited to very specific cases such as those patients who have an inability to open their mouths. with a reurgical rinse and collected in a wide-mouth jar
    - - It is essential to achieve a correct fixation to achieve results
        
        Most commonly used fixing liquids
        
        alcohol isopropilico
        
        ácido acético 2.5%. lisa hematíes y consigue frotis mas limpio
        
        alcohol de 96
        
        most commonly used, immerse the slides in a wide-mouth jar
        
        alcohol-eter
        
        50 % alcohol, 96 and eter
        
        spray polietilenglicol
        
        Sprayers with a mixture of isopropyl alcohol and a xylone
    - - 3 basic dyes
        
        orange g y ea 50
        
        Alcohols of various strengths
        
        hematoxilina de harris
        
        xileno
    - - More active cell structures have a basophilia and bluish coloration. Less active structures are acidophilic and pinkish coloration
        
        Intermediate stratum
        
        Larger, more flattened cells, smaller nucleus, blue or pink cytoplasm
        
        Superficial stratum
        
        pink cells, small nucleus and pincotic, flattened and smaller in size
        
        Parabasal
        
        flattened, large nucleus, nucleolus, bluish cytoplasm
        
        stratum corneum
        
        small bright pink to red and anucellated