Supplements comprise hydrolyzed collagen can aid in skin hydration, reduce the appearance of wrinkles, and improve skin elasticity. This can be determined by analyzing transepidermal water loss through various methods such as corneometry, moistureMeterSC probe, cutometry, silicon skin replicas with optical 3D phase-shift rapid in-vivo measurements, skin ultrasonography elasticity probe, SkinLab USB DermaLab, and histological analysis TEWL probe.