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Diagnostics for plant pathogen detection, DNA isolation, alternative to…

- - - - :check:
        precise, cheap, fast, without biological risks.
      - :red_cross:
        difficolti to have enough antibodies, ethical problems, must to know the pathogen that we want to isolate, low sensitivity problems, only for bacteria and viruses, not for detect fungi
      - Polycronal / monoclonal antibodies
      - Direct: based on the antigen recognized by the antibody bound to the enzyme
      - indirect: secondary antibody (bind to the enzyme) that recognize the primary antibody
      - Double Antibody Sandwich: strip where the antibody is already bound to the bottom of the tube, easy attachment of the antigen. antigen between 2 antibody
      - Triple Antibody Sandwich: in laboratory different step: Polyclonal Antibody, Antigen --> wash, Monoclonal Antibody, Conjugate Antibody, reagent
      - Competitive: antigen is bind with enzymes, if there is more pathogen (antigen) we have less color.
      - commercial ELISA kits
        
        Lateral flow devices or immunostrips for fungi, viruses and bacteria