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METHODS - Coggle Diagram
METHODS
Molecular Biology
Cloning
Cells
PCR
Transformations
Mini/midi preps
Constructs? maybe in apendix
Primers
Sequencing
qPCR
Cell culture
Cell lysis (maybe)
Straight into sample buffer
Lysis buffer
Lentivirus production
HEK plating and transfection
Harvesting
Adding to neurons
Cell Culture
HEKs
Neuronal culture
Dissection
Maintainance
Plate prep
Medias
Transfections
Neuronal only
Microscopy
Coverslip prep
Staining
antibodies
Confocal imaging
Microscopes
Settings
Image analysis
Mitochondrial morphology
Synapse number
Protein Biochemistry
Surface Biotinylations
Biotin, buffers
Process
Streptavidin pulldown
Western Blotting
SDS-PAGE
Transfer
Probing - antibodies
Mitochondrial isolation
Cell growing and pelleting
Abcam kit
TMT Mass Spec
RIPA Buffer
Analysis (Phil)
Mitochondrial activity assay
Promega kit
Plate set up
Buffers
in a big table
Materials