Please enable JavaScript.
Coggle requires JavaScript to display documents.
illumination(Some organism are so small that they are only visible to our…
illumination(Some organism are so small that they are only visible to our eyes under certain wavelengths)
Brightfield Illumination:
This is the most basic and commonly used illumination technique. In brightfield microscopy, light passes through the specimen, and the background is illuminated.
Best for: Transparent or lightly stained specimens like bacteria, blood cells, and tissues.
-
Darkfield Illumination:
Darkfield microscopy involves directing light at an oblique angle to the specimen, so only scattered light reaches the objective lens.
Best for: Transparent or translucent specimens, such as living cells, diatoms, and certain bacteria.
-
Phase Contrast Illumination:
Phase contrast microscopy exploits the phase differences between the direct and diffracted light passing through a specimen.
Best for: Unstained living cells, cell organelles, and other transparent specimens.
-
Differential Interference Contrast (DIC) Illumination:
Description: Similar to phase contrast, DIC microscopy enhances contrast by detecting changes in refractive index in a specimen.
Best for: Observing structures with varying refractive indices, such as live cells and tissue sections.
-
Fluorescence Illumination:
Uses fluorochromes or fluorescent dyes that emit light of a different color when exposed to specific wavelengths.
Best for: Fluorescently labeled specimens, including cells, tissues, and proteins.
-
Confocal Microscopy:
Confocal microscopy uses a pinhole to eliminate out-of-focus light, producing sharp, high-contrast images.
Best for: 3D imaging, studying subcellular structures, and detailed examination of thick specimens.
-
Polarized Light Illumination:
Polarized light microscopy uses polarizers to control the orientation of light waves.
Best for: Crystals, fibers, and certain biological structures like collagen.
-
