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6.1.3 Manipulating genomes - Coggle Diagram
6.1.3 Manipulating genomes
DNA profiling
what is DNA profiling and how does it generate a genetic fingerprint?
uses of DNA profiling (at least x2)
method for DNA profiling (stages 1-6) and explanations for each stage
which way is the gel run and why? which are the smallest vs largest fragments?
PCR
what is needed for the PCR reaction mixture (x4 'ingredients')?
what are the stages that a thermal cycler use for each cycle?
what is PCR used for?
how do you calculate how many fragments will be produced after 9 cycles?
DNA sequencing
what is needed for the DNA sequencing reaction mixture (x4 'ingredients')?
what is DNA sequencing?
draw a series of diagrams to explain the stages of DNA sequencing (stages 1-4)
what are the new DNA sequencing techniques and what are the advantages of them?
uses of DNA sequencing
uses of
comparing genomes
1.
2.
3.
what is
bioinformatics
?
what is
computational biology
?
proteomics
synthetic biology
Genetic engineering
in
prokaryotes
draw a series of diagrams AND describe the stages of genetically modifying a prokaryotic cell (steps 1-4)
uses of genetically modified bacteria
ethical concerns of genetically modifying bacteria
in eukaryotic cells
in
animal
cells
uses of genetically modified animals
1.
2.
3.
Gene therapy
in medicine
somatic cell gene
therapy
germ line cell gene therapy
methods for genetically modifying animal cells
1.
2.
ethical concerns of genetically modifying animal cells
in
plant cells
draw a series of diagrams AND describe the stages to genetic modify plant cells to become insect-resistant using Agrobacterium tumefaciens?
what are the advantages of genetically modifying plants?
what are the issues relating to patenting and technology transfer e.g. making genetically modified seed available to poor farmers?
ethical concerns of genetically modifying plants
what are the principles of genetic engineering?
