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4.3 DNA Sequencing - Coggle Diagram
4.3 DNA Sequencing
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Sanger Method
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template DNA supplied w mix of all dNTPs, primer sequence complement to 3' end
diff tube of all four dideoxynucleotide present in limit quantities and label w tag that fluorescence
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chain termination, primer has defined length of 32P at 5' end and hybridize at 3' end of template strand
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running the product in each tube-on a gel allow to determine each chain terminating ddNTPs was incorporated
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