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For the study, power analysis was applied and 4 groups of 8 rats each were…
For the study, power analysis was applied and 4 groups of 8 rats each were determined.
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For intestinal permeability analysis; a total of 32 rats were given 4 kDA fluorescein isothiocyanate-dextran (FITC-Dextran) in phosphate buffered saline (PBS) at a dose of 400 mg/kg 4 hours before sacrification by gavage
SACRIFICATION
Intestine samples for histological examination. Intestinal permeability analysis, Anti-Zo-1 immunoreactivity H-Score
Histological examination. Intestinal permeability analysis, Anti-Zo-1 immunoreactivity H-Score:
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Blood sampling for the measurement of AST, ALT, ALP, amylase, lipase, urea, creatinine and LDH in serum.
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Samples for culture for the evaluation of bacterial translocation from the liver, small intestine mesentery, and spleen
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