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PRIMARY TECHNIQUES BASED ON THE MARKER USED - Coggle Diagram
PRIMARY TECHNIQUES BASED ON THE MARKER USED
RADIOIMMUNOASSAY
RIA
Characteristics
Heterogeneous
Competitive
Marked Ag
Marker: Tritium
IRMA
Characteristics
No-comptetitive (sandwich IRMA)
Ab fixation
Heterogenous
Competitive (inhibition IRMA)
Ag fixation
Marked Ab
Radioactive marker
ENZYME IMMUNOASSAY (EIA)
Ab-Ag complex
labeled with
Enzyme
Colored compound (spectrophotometer)
Biotin-streptavidin system
Multiplies enzyme action
Homogenous EIA
Marked Ag
Competitive
More Ag (sample)
More color
More enzyme action
More marked Ag
Less color
Enzyme inhibition when binding to Ab
Heterogenous EIA
ELISA
Marked Ab
Direct ELISA
Ag fixation
Color
Ab-Ag complex
Indirect ELISA
Ag fixation
First Ab: not marked
Ab-Ac complex
binds with
Second Ab: marked
Sandwich ELISA
Ab fixation
Add Ag (sample)
Second Ab: marked
DAS
Second Ab: not marked
Third Ab: marked
HADAS
Ab fixated and marked are different
Marked Ag
Competitive ELISA
FLUOROANALYSIS
Fluorescent complexes
FPIA
Competitive
Homogenous
Marked Ag
Labeling molecule is fluorescent
Detection of the polarized light
Signal generation
Fluorescence
Molecular rotation in the solution
Polarized light
LUMINISCENT IMMUNOASSAY
MEIA
Marked Ab
Solid phase
Latex microparticles
CMIA
Marked Ab
Solid phase
Magnetic microparticles