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Sugar Plan - Coggle Diagram

- - - - other protein : Helicase、SSB
      - Synthesis_Polymerase
      - Nciking_Endonuclease
      - clean up
        
        duplex-specific nuclease (DSN)
        
        RNA precipitation
        
        Lithium polychloride precipitation
        
        Ammonium acetate
        
        Sodium acetate
        
        HPLC
    - - FFT(N3-Dye-dTTP) :red_cross:
      - N3-dTTP
        
        DBCO-Biotin :red_cross:
        
        DBCO-Cy5 :red_cross:
  - - - IVT :warning:
        
        Template Annealing :check:
        
        label N3-dNTP+DBCO-Cy5 :check:
        
        label N3-dNTP+DBCO-Biotin :question:
- - - - length
        
        123nt :check:
        
        365nt :check:
      - clean up_Primer+dsDNA
        
        HA :red_cross:
        
        PCR clean up :red_cross:
        
        Removal kit :red_cross:
        
        Linear acrylamide :red_cross: