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Genital Cultures, : - Coggle Diagram
Genital Cultures
Pre Analytical Factors
Clinical Presentation
Male
- Urethritis
- Epididymitis
- Prostatitis
- Genital ulcers
Female
- Cervicitis,
- Vulvovaginitis
- Urethritis
- BV
- Salpingitis (PID)
- Endometritis
- Genital ulcers
- Pregnancy screenings
- IUD infections
Specimen Collection
Female
Swabs of cervix, LN, urethra, vagina, and/or vulva
Male
Swab cultures of penile lesions, urethra, pub/abscesses, or LN
Order of Inoculation
- BAP
- CHOC
- MS
- MTM
- MAC or EMB
Materials
- Inoculation plates
- Selective media for yeasts
Selective Media for Yeasts
- CHROMagar
Transport of Specimen
- Swab in amines tubes
- Place transport on ice or fridge until or during transport
:warning: when do we reject samples? :warning:-
Analytical Factors
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Basic Workup of Cultures
- Inoculation
- Direct smear
- Incubation :lock:
- Culture exam
- Susceptibility testing if ordered
- Report out results
:unlock:
- 35 C in a 5% CO2 incubator
- Observe growth at 18-24 hours but hold for 72 hours
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Gram Positive Organisms
Orgs that are NF
Lactobacillus
Staphylococcus aureus
Streptococcus viridans
Streptococcus agalactiae
Listeria monocytogenes
Gardnerellea vaginalis
Bifidobacterium*
Orgs that are not NF
Staphylococcus saprophyticus
Enterococci
Corynebacterium urealyticum
Actinomyces
Gram Negative Organisms
Orgs that are NF
Porphyromonas
Orgs that are not NF
Neisseria gonorrhoeae
Haemophilus ducreyi
Escherichia coli
Klebsiella
Enterobacter
Serratia
Proteus
Providencia
Morganella
Psuedomonas aeruginosa
Chlamydia trachomatis
Ureaplasma urealyticum
Yeast and Friends
Candida albicans, tropicalis, and glabrata
Trichomonas vaginalis
Mycoplasma genitalium :red_flag:
Mycoplasma genitalium is technically a bacteria, however it does not show up on a gram stain, and isolation clinically is rare
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