lab safety rules
dress code
safety goggles/chemical resistant safety goggle
chemical products
Heat and fire
First aid
Take some distance with your face and face tube
sharp objects
Never heat a liquid in a closer container
Notify the teacher if you have any medical problem
Googles must be worn when heat is involved
heat objects
chemical
Thermal / non puncture-resistant gloves
Report any accidents or injuries to the teacher
never mix material for fun
Asthma, allergies...
Never inhale or smell things directly from a container
specimens
strong chemicals
Proper waste disposal
tie back long hair
don't wear baggy clothing
Glassware / sharp object
don't wear jewelry that can touch heat or chemicals sources
Report to the teacher broken glass/ do not handle
Know the location of all lab safety equipment
Never put broken glass into te regular trash
wear sneakers ( not open toed shoes)
Cleanup
Fire extinguisher
When investigation completed
Cleanup your work area
Fire blanket
Safety shower
Eyewash station
any substance that might get into your eyes
Hot plate, bunsen burner
Never pick up a container that has been heated using your bare hands
might produce dangerous, possibly explosive substance
use a « wafting » motion if instructed to smell
never put materials down the sing / in the regular trash without the instructions of the teacher
always wear google
handle glassware with care
notify your teacher of chipped or cracked glassware, do not use
return all equipment to its proper place
Follow cleanup instruction
why ? Can help prevent serious/fatal injuries
why ? Working with chemicals that could harm you il they come into contact city your skin
leave all personal items outside
backpack
Purses
Jackets
Why ? They dont become contaminated
Always button your coat
try to keep the cuffs tucked into your gloves
Protect form flying debris
Just safety googles : wont protect from splash hazards
!!! Make sure to choose the most appropriate type - resistant to the chemicals you’ll be hanling
change them frequently
why ?
chemicals can leak thought overtime
or use a respirator
But by law you must first complete the proper training
How ?
ask your lab manager or instrucor
remove PPE
Be aware that you can spread chemical or biological contamination by touching items while you have your gloves
light switches
Door knobs
Phone
In the labo
Respect your colleagues
Never work alone
Never try to pick up broken glass with your bare hands, a cut or puncture caused by broken glass
After you finish the experiment wash and dry glassware
returned reagents to the storage area and clean the workbench, surface with ethanol or isopropyl alcohol
When working in a lab for the first time
Look around and identify the location of the safety equipment
why?
extinct small flames on workbenches / floors
Every lab must contain a safety shower and eyewash station
Should be tested weekly to ensure there working properly
Why?
help someone whose clothing is on fire
how ?
force the flames upward toward the head and neck area
Wrap the person in the blanket and help them roll until the fire is out
each lab should have
first kit aid
Yell if you need help
contains bandages / antiseptic for minor injuries
evacuation routes posted near the exits
If you have a problem
Immediately move to the nearest safety shower
why ? In case one is blocked
Remove the saturated clothing
Thoroughly drench the affected skin under the shower
Chemical fume hood piece
Drench your entire body and have someone call 911
if youre eyes are exposed to hazardous chemicals
it’s important to now where they are
you need to know about lab safety
The eyewash station is used for rinsing your eyes
why?
Hold your eyes open and thoroughly rinse for at least 10 minutes.
Minimize the likelihood of accidents
What ? Is a ventilated and closed work area
Why ? Protects you from toxic vapors
what to do ? Tureen on the exhaust fan and make sure the hood is venting properly
careful ! Never store chemicals and the hood and always clean and remove materials when you’re finished working
Every chemicals have a technical document
GHS
New systeme
Need to know the location
SDS
globally harmonized system of classification and labeling of chemicals
International format for SDS
Identification
Chemicals name
Description
Manufacturer’s contact information
Hazard identification
Signal words
4 types of fire
Warning
Safety symbols
Class A
Composition
Ordinary combustibles
Ingredients
Wood
First-aid
Cloth
Treatment for a person who’s been exposed
Fire-fighting measures
Paper
Instructions for containment and cleanup of spills or leaks
Class B
Handling and storage requirements
Organic solvents
Exposure controls & personal protection
Flammable liquids
Physical and chemicals properties
Class C
Appearance
electrical equipements
Odor
pH
Solubility
Stability and reactivity
Which equipement for which type of fire?
How to avoid hazardous reactions
Class B and C
Toxicological informations
Chemincals foam extinguishers
Short / long term effects
Ecological
DON'T PUT WATER ON THESE TYPES OF FIRE IT WILL MAKE IT WORSE
Disposal
Transport
Regulatory
Other informations
Not mandatory
You can electrocute yourself
Class A,B,C and D
Use dry chemicals extinguishers
Class D
Combustibles metals
Reminder!
Only use à extinguisher if you are trained for this by certified trainers in à local fire departement
Where to go?
Small flames
workbenched
Floor
Someone whose clothing are on fire
Nevers wrap someone wile they're standing
Why?
It can force the flames upward toward the head and neck
1.help the person to the floor
2.wrap them into the blanket
3.help them roll until the fire is out
4.Use the first aid kit that every lab should have
What does it contains?
Bandages
Antiseptic
It's important to know multiples evacuations routes
Chemicals fume hood
It's ventiladed and protect you from toxic vapors
Turner on the exaust fan to make sure the hood venting properly
SOP
Def
written document detailing how to safely work with hazardous materials or carry out an experiment in the lab. ( detailed and accurate )
Content of a sop
promote cleanliness at all stages.
Can vary from one lab to another
MAIN PURPOSE
DISTRIBUTION
to ensure operations are performed safely and in the proper way. It also lists the key risks associated with an activity and how the risks can be controlled.
The SOP will list who has received a copy of the SOP
to guarantee that all workers are performing tasks in the same way, which is required to get expected output from the process
click to edit
chemistry laboratory,
water laboratory,
SOP NUMBERING
SAP :Administration Department
SCP :Chemistry Laboratory
ATTACHEMENTS TO SOP
SMP :Microbiology Laboratory
The SOP can add forms, tables, data sheets, etc... SOPs
SSP :Soil Laboratory
CROSS REFERENCES
SEP :Environmental Consultancy
SVP :Validation
It gives references to other SOPs relevant to the procedure in question
If there are no cross references “N/A” should be stated under this heading.
This section is found on the first page of each SOP.
Each lab has its own SOP
INTRODUCTION
Quality manager -> responsible for signing SOP
Provides a background or history to the experiment/work done.
OBJECTIVE
Describes the main aims of the procedure.
Where appropriate the additional heading should be used for Test Methods, as required:
SCOPE
Describes what and whom the procedure pertains/relates to
Equipment / apparatus
DEFINITIONS
Reagents/standards
Safety consideration
Defines any item or abbreviation that may not be familiar to the user
Sample preservation/pretreatment….
RESPONSIBILITIES
Defines the responsibilities of the main parties
SCOPE
Testing procedure
Describes what and whom the procedure pertains/relates to
DEFINITIONS
Defines any item or abbreviation that may not be familiar to the user
RESPONSIBILITIES
Defines the responsibilities of the main parties
PROCEDURE
Sequentially numbered, logical, concise and accurate steps to describe the procedure.
Other danger
Electrical shock
Lab safety survey
General safety
Never place any chemical bottles on the floor not even temporarily
Keep the lab area clean/ free of unnecessary books
Don’t bring food, chew-gum, drink
Don’t handle lab equipment without permission
Be serious/alert / never horse around
Read direction / follow instructions
Notify teacher if any spills occur
Wash your hands before and after get in the labo
Don't apply makeup while in the lab
Safety survey
Use to proactively identify hazards and corrective action we need to take (important part of a company occupational health safety)
PPE
Wearing a lab coat
Safety glasses and gloves
No jewelry, hair tied
You may need special PPE depending on the work you're doing
Like these heat resistant gloves for opening the autoclave. -> That's PPE
Labs equipment
Should be tested and certified
To be sure it's in good working order
Fume hood and biological safety cabinets
Look similar but their functions are actually quite different
A fume hood : used to protect you when working with toxic chemicals (the air flow moves the vapors away from you)
A biological safety cabinet : used for working with infectious bacterial agent (air flows through a HEPA filter to create a clean working environment, so it's protect you and you work from contamination).
Emergency gear
Know the location of your safety shower and eyewash station, fire extinguisher, and first aid kit
These areas must be kept clear of obstacles (can go get to them fast)
Safety equipment also has to be regularly inspected and tagged
Be familiar with the emergency evacuation plan, and exits should be clear and marked
Chemical safety
All chemicals in the lab must be labeled and organized
Labels provide important safety information
chemicals properties
the manufacturer
hazards
PPE
storage requirements
More details about chemicals by looking at the safety data sheets
Housekeeping requirements
Always clean up after yourself
The lab must be organized and free of clutter
Walkways should be clear of obstacles
Dispose biological wastes in the container labeled with the appropriate biohazard symbol
Clean your equipment and workbench after each experiment
Always place sharp objects like broken glasswear in a sealable puncture proof container
Burns
Gaz cylinders
Slips / Trips/falls
Why ? Many peace’s of equipment operate at very high voltage
Keep water/ liquids away from instrument
Never attempts to repair or open any cover while instrument plugged in.
Routinely check for frayed electrical cords
Only use approved extension cables or power switches
Why ? many lab instrument operate at high temperatures and can cause burns
use heat-resistant gloves whenever handling hot materials
Extremely low temperature can also damage unprotected skin
wear insulated gloves when handling dry ice / items in the -80C° freezer
can cause very serious injuries
keep floors and routes of movement through the lab
never place any materials or instruments on the floor
clean up spills immediately + use « wet floor »signs to warn colleagues
be aware of surroundings : look out for shelves + tables tops + any other structures that can cause a head injury if you bump into them
extremely flammable
secure gas cylinders with a heavy-duty clamp and strap
the cylinder’s head should be sealed with a threaded protective cap whenever it’s not in use