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Artificial Meat Cultivation Techniques - Coggle Diagram
Artificial Meat Cultivation Techniques
Scaffolding
Micro carriers
Could be employed as temporary carrier to support cell proliferation (non edible), release cells (non edible) or incorporated into final product (edible)
Advantages: simple to produce & allow efficient scale-up of adherent cells
Disadvantages: Nonedible micro carriers would require an additional dissociation step which would increase overall costs & introduce safety concerns
Porous scaffolds
Possesses sponge-like structure which provides mechanical stability required for seeded cells to form tissues & deposit ECM
Advantages: cheap and easy to produce,
Disadvantages: technique may not be useful for production of whole cut meat products
Fibrous scaffolds
Nanofibers produced through spinning techniques
Advantages: ideal for inducing muscular fiber alignment, relatively straightforward to produce, can be applied to variety of biomaterials
Disadvantages: difficult to scale up
Hydrogels
Advantages: mimic structure of naturally occurring ECM which aids cellular compatibility, require little scaffold material
Disadvantages: certain types of hydrogels may affect cell proliferation, motility and differentiation
3D bio printing
Advantages: can produce defined structures at variety of scales with precise control over positioning of cells & bioinks
Disadvantages: trade off btw printing resolution and speed
Stem Cells
Adult Stem Cells
Culturing adult stem cells in collagen matrix obtained from live or dead animals with necessary sources of energy required for cellular proliferation & differentiation
Advantages: rapid growth rates as compared to conventional meat sources
Disadvantages: not effective in energy, water and raw material usage, limited commercial applications, animal origin culture medium may still be used
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Self Organising Techniques
Relatively limited to research as of now
advantages: can be used to create highly structured meats
disadvantages: lack of blood circulations inhibits substantial growth