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chromatographic Techniques - Coggle Diagram
chromatographic Techniques
column chromatography
working
application
helpful in seprating compound mixture
used to imrove imprities
isolate metabilites from biological flid
advantage
ant type of mixtre can be seprated
any quantity of mixture be seprated
wider choice of mobile phase
limatation
time consming
more amount of mobile phase required
atomatio make it more complicated & costly
paper chromatography
working
advantage
cheap
efficient for polar & water soluble compond
easy to handle & store
application
in sepration of biological sample
in preparative metod
in field of food drug and cosmetics
analysis of blood, and diagnosis value
in field of biochemistry
limatation
large qantity of sample cant be applied on paper
complex mixtre cant be seprated
less accurate
thin layer chromatography
working
application
sepration of individal component of mixture
checking purity of sample
prification process
identification of reaction
advantage
easy to perform
no instrument required
low cost
various staining process possible
limitation
experties required
qualitative analysis
loss of charged
Adsorption chromatography
working
application
isolation of antibiotics
identification of carbohydrates
seprate & identify fats
high performance liquid chromatography
working
application
precise way to check purity
used in pharmaceutical labs
used in food, botanical labs
used in forensic &drug development
advantages
high speed in minutes
accuracy
high resolution
limitation
costly, complex to operate
doesnt work or all samples
need a skilll to run
ion exchange chromatography
working
application
sepration of protein from food
demineralization of water
measre additives in food
concentrate metal ion in sample
advantages
resolve complex samples
detection of many inorganic salt
detection of organic ion
limitation
column efficiency is less
difficult to control
sepration of on;ly charged molecule
