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Fluorescence Microscopy, 220px-FluorescenceFilters_2008-09-28.svg, NAME:…
Fluorescence Microscopy
Working Principle
1- Light source
Mercury lamp
Xenon lamp
2- Excitation filter
permits desired wavelength of light only
3- Dichroic mirror
Reflects the excitation light
4- Fluorophores
absorb light
Electrons reach excited state
electrons emit light at Ground state
longer wavelength light
5- Objective lens
magnify image of sample
collects emitted light
6- Emission Filter
permits emitted light only
blocks excitation light
7- Ocular lens
final magnification of image
light passes to detector
8- Detector
Detect light
9- Camera
Advantages
High Sensitivity & specificity
Live cell imaging
High resolution
Disadvantages
Photo bleaching
Phototoxicity
Background noises
NAME: MAHNOOR BAIG
