Please enable JavaScript.
Coggle requires JavaScript to display documents.
YbAc-pH - Coggle Diagram
YbAc-pH
Create a symbolic link
Use the terminal command: ln -s /mnt/emstorage/facility/stain-proj/Eu-zero/ .
Relion: Import tiff images
Job: 001
Import: *mrc
Pixel size: 3.75
Voltage: 120
Spherical abberation: 2
Amplitude contrast: 0.1
Output
81 micrographs
Manual picking
Job: 002
Particle diameter: 500
Output
904 particles picked
Extract particles
Job: 003
Particle box size (pix): 120
Re-scaled particles size(pix): No
Output
904 particles
2D classification for template
Job: 005
CTF correction: Yes
Ignore CTFs until first peak: No
Number of classes: 10
T: 2
Iterations: 25
Mask diameter (Å): 500
Image alignment: Yes (6-5-1)
2D Helical segments: No
Output
Auto-refine: Estimated accuracy angles= 3.85 degrees; offsets= 6.45 Angstroms
CurrentResolution= 32.1429 Angstroms, which requires orientationSampling of at least 7.34694 degrees for a particle of diameter 500 Angstroms
Select classes for template
Job: 007
Output
435 particles picked.
Select 10mics to test
Job: 008
Output
149 particles picked.
Autopicking
Job: 009
Low pass filter: 20
Picking Threshold: 0.5
Inter-particle distance: 220
Output
126 particles picked.
Autopicking
Job: 011
Low pass filter: 20
Picking Threshold: 0.5
Inter-particle distance: 220
Output
10,058 particles picked.
2 more items...
Autopicking
Job: 009
Low pass filter: 20
Picking Threshold: 0.8
Inter-particle distance: 220
Output
91 particles picked.
Not used further..
