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RT-PCR - Coggle Diagram
RT-PCR
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Disadvantages
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If standards are made incorrectly, quantities of samples will also be incorrect
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Evolution
Initially, focused on amount of human DNA in sample
Next, they quantified amount of male DNA which helped with STR analysis
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Detects and measure exponential accumulation or reduction of light emitted from fluorescent dyes as rxn progresses
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Most accurate, precise, and efficient method for human DNA quant