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Mass
spectrometry - Coggle Diagram
Mass
spectrometry
basic concept
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- TIC: one acquires a mass spectrum over a wide range of m/z ratios (50 to 2000). after the computer sums all signals at all m/z values in each spectrum.
- SIM: you set the MS acquisition in a narrow m/z interval (<10 u), which contains that of the analyte of interest.
- EIC: a SIM-track-like chromatogram can also be constructed from a TIC acquisition by requiring the computer to extract from the total MS spectra only the intensity relative to the ion with the desired m/z ratio.
CALIBRATION
external standard
The sample is enriched with one or more known amounts of a standard solution of the analyte. Measurements are made on the sample and the sample in which the standard analyte solution has been added, then the responses are used to calculate the analyte concentration, assuming a linear relationship.
internal standard
A known amount of a reference species is added to all samples, standards and blanks.
Than the analyte/reference signal ratio is reported as a function of analyte concentration in the standards.
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