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Literature Search, Online databases, Why management is needed!, in silico…
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Online databases
Google scholar
pros
You can find anything
High tolerance to spelling mistakes
cons
Search algorithm is not transperant: bias?
not all refs are peer reviewed
Citaion might be misleading
PubMed
covers publications since mid 1940s
Over 5,500 journals are indexed
Biased towards medical papers
Web of Science
including tools for Science Citation Indexing
How often was a paper or author or journal cited?
Who cited which work?
Researcher and journal performance.
Use firefox
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Colony PCR
Design primers
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pros: Provides informations about the size of the insert and its presence.
Can be used to screen clones that were created with same backbone because primers are plasmid dependent.
cons: Doesn't tell orientation
pros: Tells the orientation
Tells you if the insert is present
great for blunt end cloning.
cons: You have to make a new primer pair for each insert since primers are insert dependent
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Bolean Operators
Pto DC3000 AND* effectors (have both terms in them)
Pto DC3000 OR effectors (all papers have one of these terms)
Pto DC3000 NOT effectors (all papers with pto DC3000 without effectors)
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Software
Notes clearly assigned to one source
Work with digital publications
Evaluate & edit literature
Work systematic
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