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Nucleus - Coggle Diagram
Nucleus
DNA
Histones & nuclear proteins
DNA wrapped around histone (8 protein molecules)
Nucleosome = DNA &histome complex
Non-histone protein
Chromatin
Complex structure of protein and DNA in mitotic cells
provides info.
Cell type
Differentiation
Proliferation status
Malignancy
Chromatin fibres condense into chromosome
cell divide
Two morphologically distinct states
Euchromatin
contains transcriptionally
active protein-coding DNA regions
(
pale
staining)
Heterochroatin
closely packed DNA where
transcription X occur
(
Dark
staining)
Haematoxylin (with AI mordant)
bind negative charge of DNA
visualisation of chromatin pattern
Staining intensity
Hyperchromatic
(darker staining)
more chromatin, size available for Haematoxylin
Cell nucleus shrink as chromatin coming tgt
Normochromatic
(normal staining)
Hypochromatic
(pale staining)
Cell swelling (degenerative)
Structure
Granularity
Distribution
Nucleolus
Region of ribosomal subunit preparation
Size and prominence related to protein synthesis
Darker staining area
Eosinophilic
High protein content
Basophilic
High RNA content
Look for magnlicancy (reactive change)
Nuclear membrane
Bi-lipid membrane (not visible by LM)
Chromatin connected to inner membrane at many point
Membrane visualised bacsue of the chromatin