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microscopy (strand 2) - Coggle Diagram
microscopy (strand 2)
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electron microscope
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gives a high resolution so organelles such as mitochondria, ER and membranes are seen in detail
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practical
- stain sample with appropriate dye
- mount sample using a pipette to put a drop of water onto the slide
- using forceps place the sample onto the slide and use a mounting needle to carefully place the cover slip on top
- clip the slide onto the stage
- select the lowest-powered objective lens
- use the coarse adjustment knob to move the objective lens to just above the slide
- look down the eyepiece and adjust the focus by moving the lens away from the slide using the fine adjustment knob, until a clear image appears
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