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Revision, Polymerase DNA chain, Polymerase DNA chain, Replication of DNA,…
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Polymerase DNA chain
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- DNA
- Primers
- Supply of free DNA nucleotides
- Heat tolerant DNA polymerase
- pH buffers
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Replication of DNA
- DNA is unwound and the hydrogen bonds are broken to form template stands
- DNA polymerase add free DNA NUCLEOTIDES using complimentary base pairing, to the deoxyribose 3' end of the new DNA strand which is forming
- DNA polymerase can only add nucleotides to a chain that already exists so a primer is needed
- DNA polymerase can only add nucleotides in one direction resulting in a leading strand being replicated constantly, the lagging strand is replicated in fragments
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