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Factors Affecting AEX Enrichment - Coggle Diagram
Factors Affecting
AEX Enrichment
interference from
Process related Impurities
Reduce impurities
using flocculation (works well)
Reduce impurities
before AEX (demonstrated but not
implemented due to
good performance of flocculation)
Full% in affinity pool
Increase Full% in bioreactor
Mitigate Full% loss
from harvest to affinity step
Flocculation (low pH)
DF (D0HC Cellulose membrane)
MCL (temp.)
TFF1 (PES membrane)
Affinity (pH 3 elution)
Interference from
Product Related
Impurities
(Deamid, Phos, etc)
Prevention
Downstream controls (neutral pH, shorten holding time)
Phos Inhibitors?
upstream production (temp, pH, duration, etc)
Removal
2nd AEX Reprocess (not work well since retained empty vectors are AEX strong binders (suggestion more neg. charges on these empty vectors))
Orthogonal Separation
Same AEX with pH elution
CEX (Poros XS)
MMC (PrimaS)
HIC (OH)
Conservative AEX Peak Cutting
(not effective when F% <10% in Aff. Elu)