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(structure of dna, light absorption of dna and rna, denaturation of dna,…
structure of dna
nitrogenous base
G to C-3 H bonds
having 3 H bonds mean if there is higher GC% content temperature used to melt the dna will be higher
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G and A are purines, C and T are pymiridines
C+T=A+G but G+C does not = to A+T, C=G, A=T
antiparallel strands connected by the hydrogen bonds between complementary nitrogenous bases in a double helix structure
one strand goes in the 5 to 3 direction, the other goes in the 3 to 5 directiin
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phosphate group
forms phosphodiester bonds with the other nucleotide by attaching to the 3'OH on the other nucleotide
backbone is the pentose sugar and phosphate group, nucleoside is the pentose sugar and nitrogenous base
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denaturation of dna, methods and factors
melt by temp, higher than melting temp
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low ionic strength of solution weakens the h bonds, lowering the melting temp
concentration, ionic strength of solution
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rna typically single stranded, involved in gene expression, does not serve as storage for genetic material for eukaryotes and prokaryotes