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Populations Practicals - Coggle Diagram
Populations Practicals
Haemocytometer
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Calculating
Cell Density
- suspension containing cells
placed on grid
- depending on cell density
use type A,B,C squares
3.count using NW rule
- count cells lying on N + W line
Important Points
- MIX yeast
cell suspension
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- for sampling natural environments
- same DEPTH
- same TIME OF DAY
- avoid yeast on TOP of COVERLSIP
or into GROOVES of haemocytometer
- carry out appropriate number replicates for reliability
Serial Dilution
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method
- 1cm3 of original suspension
- add 9cm3 of isotonic buffer
- new solution 1/10th dilution
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Mark, Release, Recapture
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Method
1.LARGE sample of species CAUGHT using:
2.caught animals MARKED
- must last over sampling period
- permanent or semi-permanent
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4.marked animals RELEASED
- sufficient time sample to mix though overall population
5.population RE-SAMPLED
- using same trapping process as before
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