Please enable JavaScript.
Coggle requires JavaScript to display documents.
Immunity Practicals - Coggle Diagram
Immunity Practicals
Aseptic Technique
D: prevent contamination when
working with microorganisms
prevent contamination of both:
microbe culture
individuals working with culture
culturing microorganisms
solid agar
liquid broth
Transferring
Microorganisms
Metal Inoculating
Loop
FLAME LOOP
hottest part Bunsen flame
until red hot
air-cool loop
after transfer microbes - RE-STERILISE
Plastic Disposable
Loop
discard in solution of
disinfectant
Culture Bottle to
Petri Dish
lid held same HAND
holding loop
lid not touch BENCH
AFTER REMOVING + BEFORE REPLACING LID
STERILISE lid region -
NECK culture bottle
through Bunsen flame
lid Petri dish only raised
small for sugar to be added
Petri Dish
labelled outside
of base
incubated UPSIDE down in
incubator @ appropriate temp
Cells in Suspension
SPREAD PLATE
L-shaped spreader
Effect of Different Antibiotics
on Bacteria
agar inoculated with bacterium
place discs of different antibiotics on agar
E-Strips
D: prepared strips that contain concentration gradient of antibiotic running along length of strip
method
place strip on agar
incubate
min conc antibiotic inhibit bacterial growth
Preparation of Streak Plate
to Isolate Single Colonies
1.
SPREAD MICROORGANISM
using inoculating loop
over small section agar on PD
2.
STERILISE LOOP
STREAK
several lines of MO
ACROSS
agar plate @
ANGLE
90°
do not allow separate lines to overlap
make
FINAL STREAK
5.
INCUBATE PD
@ suitable temp
now able identify isolated pure colonies
Antimicrobial Properties
of Plants
produce compounds to
reduce
fungal/bacterial growth
wild garlic, mint, bluebell
method
1.
GRIND
up leaves each plant
ADD WATER
in separate mortars
prepare
AGAR PLATES
+ spread
FUNGUS/BACTERIUM
culture on PD
using appropriate
aseptic techniques
3.
SOAK
small disk of
filter paper
with each PLANT EXTRACT
place on AGAR in PD
4.
INCUBATE
24-48hrs
compare results in different PD