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CRISPER CAS 9 - Coggle Diagram
CRISPER CAS 9
APPLICATION
FORM ANIMAL
disease resistant
improved fiber quality
improve meat quality
milk improvement
muscle mass improvement
healthier animal-derived product
FISHRY PRODUCTS
gene function identification
human disease modeling
improve meat quality
mussel mass improvement
PLANT CROP
bioactive compound production
biofuels development and production
crop production improvement
disease resistance
improve quality
physical and chemical resistance
therapeutic application of CRISPER CAS 9
genetic blood and lung disease
blindness
mitochondrial disease
cancer
genetic liver disease
virus infection (AIDS, COVID-19)
Advantages
precise gene repair
permanent gene knock out
target multifil gene simultaneously
help develop transgenic animal disease model
CRISPER sgRNA honing mechanism ........easier gene editing
limitation
undesired mutation ...... due to non-target effec
t
variable efficiencies of sgRNA
Low homology directed repair efficiency
PAM size limitation
large protein size...... difficulty in gene delivery....... hinders editing efficiency
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