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CRISPR-Cas9 CRISPR, SUBMITTED TO: DR WAJEEHA SAEAED
SUBMITTED BY: NAUMAN…
CRISPR-Cas9 
Therapeutic Application
In humans
Cancer: ovarian cancer. Brain cancer. urinary Bladder Brest cancer. Cervical cancer
Viral Infection.
Oncogenic virus Hepatitis B virus Hepatitis C virus
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In Plants
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Developing biotic and abiotic resistance traits in crop plants
edit crops without having other genes
In Animals
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To induce extensive DNA modifications
Commercial Application.
Biomedical
Gene Therapy. sickle cell disease B Thalassemia, Hemophilia A, Hemophilia B
Genome Editing
Industrial
Biofuel, Pharmaceutical, Crop nutrition.
Agriculture
Pest Control, Crop improve, livestock.
Advantages
create genetic variants for desired locus, allow multiple gene editing 2 or more sgRNAs, introduce homogenous mutation in 1st generation.
target specific, multi potential, cost effective, less time requir, PAM expansion
Disadvantages:
lack of basic tools genome instability off target effect bigger protein size
LOW HDR EFFICIENCY
SUBMITTED TO: DR WAJEEHA SAEAED
SUBMITTED BY: NAUMAN KHAN
ROLL NO: F22-1165
MPHIL BIOCHEMISTRY
DEPARTMENT OF BIOLOGY
UNIVERSITY OF HARIPUR