CRISPER CAS 9

Histry

Advantage

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commercial application

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From Escherichia coli bacteria

first discover in sequence of DNA

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Therapeutics

described in 1987

cost effectiveness

high accuracy

simplicity

multiplexing

quickly create animals model

directly applied in embryo

damage DNA

Limitation

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incomplete editing

inaccurate on target or off target editing

lack of on target efficiency

unwanted changes in human embryo

not always efficient

may initiate mutation

In animals

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animals grows bigger and faster

produce edited pigs

potentially promote tolerance in pigs

manipulate the genome of organism

preventing hyper acute rejection

In plants

improve quality and nutrational value

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used in growing monocot and dicot plant species

defend against pest

enhance tolerance to biotic and abiotic stress

first use of crisper in plants in 2013

In human

T cell can reveal the gene regulatory network in primary cell

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inherited eye disease

such as inherited blood disorder

understanding complex regulatory network in TME

treated many human genetic disease

explore the function of cancer related gene

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discover and develop potential new treatment and genetic cause of human disease

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established partneeship with other compnies

research develop and commercialize medicine

having several drugs in development for blood disease:

used to ameliorate disease

allow Dna to specifically modified and edited

rare blood disorder