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Extraction loss evaluation, Feces test: Samples overview, Private…

- - - - Dry up
        
        Add 3 ml of MeOH to remove the residues from the vial wall
        
        Transfer to 4 ml vial and dry up
        
        Redissolve in 50 μl 0.1% aqueous FA + 30 % ACN
        
        LC-MS
- - - - DW-U-F0
        Toluene
        
        DW-U-F1
        Tol:MeOH 1:1
        
        DW-U-F2
        MeOH
- - - - Dry an
- - - - 0.1 g of sample mixed with 2 g of sand and placed to exctraction cell
        
        Dried up
- - - - Dry up
        
        Add 3 ml of MeOH to remove the residues from the vial wall
        
        Transfer to 4 ml vial and dry up
        
        Redissolve in 50 μl 0.1% aqueous FA + 30 % ACN
        
        LC-MS
- - - - Dry up
        
        Redissolve in 100 μl 0.1% aqueous formic acid with 30 % ACN
        
        LC-MS
- - - - Present your project
- - - - Neutral fraction
        
        Fractionation
        
        Hexane
        
        DCM
        
        Silylation
        
        GC-MS
        
        DCM/ acetone (2:1, v/v)
      - Acidic fraction
        
        Acidified to a pH<2
        
        10 mL water, LLE with CHCl3 (3x15 mL)
        
        Methylation
        
        LLE with
        3x1 mL hexane
        
        Fractionation
        
        3 more items...