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BIO359 Lab - Coggle Diagram
BIO359 Lab
Lab 6
Internal size standard
Spaces fragments of 60-200bp every 20 bases
Spike
Artifacts caused by voltage exhibiting an unusually thin peak shape
DNA Mixture
Presence of more alleles resulting from more than one individual contributing to a sample
Peak Height Imbalance
A greater than 30% difference in the heights of two peaks
Pull-up
Failure of the software to discriminate between the dye colours
Stutter Peak
Small peaks that occur immediately before or after a real peak
Lab 7
Sequencing cytb
If the species is a non-protected common species
If the sample is from a related species
If the sample is from a particular species
High inter specific and low intra specific variation
DNA purified using QIAmp kits are up to 50kb in size
DNA Extraction
Lyse
Bind
Wash 1
Wash 2
Elute
AGRF
Full sanger sequencing process (Purified DNA)
QIAmp DNA Mini
Lab 4
Quantitative Polymerase Chain Reaction (qPCR)
Measuring amount of DNA in a sample
Identifying presence of inhibitors
Dilution
To dilute a DNA sample, what quantities of DNA & water would you add to generate a 1:15 dilution?
1ul of DNA and 14ul of water
Master Mix
Components
Reverse primer
Water
KAPA Sybr Green 2x
Forward primer
Lab 5
STR
Predictably inherited
Short repetitive sequences of DNA
Amplification components
Nuclease-free Water
Gold St*r 10x Buffer
PowerPlex 16 10x Primer Pair Mix
AmpliTaq Gold DNA Polymerase