Look at the Vehicle wells

Cells Turned from purple (Strain A) to yellow (Strain B):
Plate should be disregarded as not reliable; possibly due to contamination

Cells stayed purple in both Strain A to Strain B:
Plate is reliable can carry on analysis

Look at wells containing 9-AA (positive control for TA154)

If a significant amount of the cells in the plate turned from purple in Strain A to yellow Strain B it indicates that a Frameshift mutation has occurred

If a significant amount of the cells in the plate remained purple from Strain A to Strain B it indicates that no frameshift mutation has occurred

Look at wells containing N4-AC (positive control for TA100)

Look at wells containing N4-AC (positive control for TA100)

If a significant amount of the cells in the plate turned from purple in Strain A to yellow Strain B it indicates that a Base pair mutation has occurred

If a significant amount of the cells in the plate remained purple from Strain A to Strain B it indicates that no Base pair mutation has occurred

If a significant amount of the cells in the plate turned from purple in Strain A to yellow Strain B it indicates that a Base pair mutation has occurred

If a significant amount of the cells in the plate remained purple from Strain A to Strain B it indicates that no Base pair mutation has occurred

Compound of interest caused both a frameshift and a base pair mutation

Compound of interest causes only a frameshift mutation

Compound of interest only causes a frameshift mutation

Compound of interest does not cause either a base pair mutation or a frameshift mutation

Look at wells containing the test compound

Look at wells containing the test compound

Look at wells containing the test compound

If most of the cells are yellow in strain B of the +S plate we can determine that the test compound requires activation to cause the mutations

If most of the cells are purple in strain B of the +S plate we can determine that the test compound does not require activation to cause the mutations

If most of the cells are yellow in strain B of the -S plate we can not determine whether the test compound requires activation to cause the mutations.

If most of the cells are yellow in strain B of the +S plate we can determine that the test compound requires activation to cause the mutations

If most of the cells are purple in strain B of the +S plate we can determine that the test compound does not require activation to cause the mutations

If most of the cells are yellow in strain B of the -S plate we can not determine whether the test compound requires activation to cause the mutations.

If most of the cells are yellow in strain B of the +S plate we can determine that the test compound requires activation to cause the mutations

If most of the cells are purple in strain B of the +S plate we can determine that the test compound does not require activation to cause the mutations

If most of the cells are yellow in strain B of the -S plate we can not determine whether the test compound requires activation to cause the mutations.