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Biology of STRs, QPCR, Amplification - Coggle Diagram

- - - - Relative DNA amount
      - Fold change in expression level
    - - Copy number
      - Viral titre
  - - - Cost effective
      - Easy to use
      - Easy to design
      - Easy to analyse
    - - Low fluorescence
      - Bound to dsDNA, fluorescence increases 1000 fold
      - As amount of ds DNA increases, amount of dye that can bind increases
      - Con: Non-specific binding
      - Reaction specificity determined by primers