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DEHYDRATION - Coggle Diagram
DEHYDRATION
DEHYDRATING AGENTS
Alcohol
Ethyl
Methyl
Butyl
Utilized in plants and animal micro-technique
Slow dehydrating agent
Produces less shrinkage and hardening than Ethyl
Recommended for tissues which do not require rapid processing
Boiling Point: 117.7 degrees C
Toxic Dehydrating Agent
Primarily employed for blood and tissue films and for smear preparation
Boiling point: 78.3 degrees C
Recommended dehydrating agent
Not poisonous and not very expensive
Ethanol (Ethyl Alcohol)
Non-Toxic
Miscible n all portions of water
Little Shrinkage if graded alcohols are used
Can be used on Eyed and Embryos
Acetone
Rapid in action but penetrates tissue poorly
Most lipids are removed from tissues
Extreme Volatile and Inflammable
Boiling Point: 56 degrees C
Dioxane
Diethylene Dioxide
Dehydrating and Clearing Agent
Produces less tissue shrinkage
Tissues can be left in this reagent for a long time
Tissue section dehydrated with dioxane tends to ribbon poorly
Extremely Dangerous - main disadvantage
its vapor produces a cumulative and highly toxic action in man
Refractive Index: 1.42
Boiling Point: 101.5 degrees C
Cellosolve
Ethylene Glycol Monoethyl Ether
Triethyl Phosphate
Tetrahydrofuran
Removes Intercellular and Extracellular water from the tissue.
Follows Fixation but before Infiltration