Evidence: In the butterfly experiment I saw on the graph that the lighter colors had more allele frequency than the darker colors because of blending. The lighter colors usually spiked in the fourth and fifth generations then went back down. My color of butterfly was white, and I think that if the generations would have kept going, we would've kept getting less and less allele frequency because we were losing a lot of butterflies as the generations went on, and we got hunted. These allele frequencies change from multiple factors one of these factors being gene flow. In gene flow, certain animals move in and out of a population, and after a while, they will bring new traits with them. Or if a new type of animal from other areas comes, they can mix their genes, changing the allele frequency which increase genetic variety. This step can then lead to separation. Separation can lead to new species through a few important steps. First, animals from the same group must stop breeding and be separated from each other. This prevents the genes from mixing. Next, there need to be different mutations that create different traits. Third, a long period of time needs to pass so the traits can develop and change. Finally, over time, natural selection will favor those traits and help them survive in their environments. And then, when they are reunited, they won't interbreed.