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Materials and Method - Coggle Diagram

- - - - hydroxyethyl starch (HES) at 1:6 ratios
    - - centrifuging them at 300xg for 10 min at room temp
      - Ficoll-HyPaque(Pharmacia-Amersham, Piscataway, density 1.077 g/ml)
    - - low-density mononuclear (MNC) jointing layers
      - phosphate-buffered saline (PBS)/EDTA [32]/*2
    - - magnetic cell sorting (MACS) technology
      - (Miltenyi Biotec, CA, USA)
    - - 100 µl blocking solution along with 100 µl CD133+ microbeads
      - (Miltenyi Biotec, CA, USA)
      - for 1 h at 4 °C in dark.
    - - the incubated cells using CD133+ microbeads
      - at 300×g for 5 min at 4 °C
      - resuspended in PBS buffer
    - - LS columns
    - - purity of the isolated CD133+ cells
      - Flow cytometry analysis