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Microbio Ch. 2, 3, & 6 - Coggle Diagram
Microbio Ch. 2, 3, & 6
Ch. 2
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Staining
Simple
Acidic
(-), repelled by (-) cell wall
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Basic
(+), attracted to (-) cell wall
Methylene blue, Crystal violet, Safranin
Differential
2+ dyes, most common, distinguishes structures
Gram stain
(+) = thick peptidoglycan (purple, blue)
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Acid fast
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M. tuberculosis, M. leprae
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Ch. 3
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Membrane
Gram neg. (outer)
-
-
Lipid A: endotoxin (fever, shock)
-
-
Membrane transport
Passive (no energy)
-
-
Osmosis
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Tonicity
Hypertonic: Water leaves the cell, shrinkage
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Hypotonic: Water enters the cell, swelling
Active (ATP required)
-
-
-
-
Bulk transport
Endocytosis: Phagocytosis, Pinocytosis, Receptor-mediated
-
-
Internal structures
Cytoplasm: 80% water, dissolves macromolecules
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Ribosomes: 70S, protein synthesis target for antibiotics
Inclusions: Storage of nutrients (polysaccharides, sulfur, gas vacuoles)
Endosymbiotic theory
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Mitochondria + chloroplasts: 70S ribosomes, contain own DNA, binary fission
External structures
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Flagella (motility)
Filament, hook, basal body
-
-
Fimbriae: Attachment to cells & surfaces, aids in colonization
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Ch. 6
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Viral Structure
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Capsid: protein coat, capsomeres
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Morphology
Polyhedral
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Ex: Poliovirus, Adenovirus
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-
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