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IRON SUCROSE - Coggle Diagram
IRON SUCROSE
IRON CORE CHARACTERIZATION
2.CRYSTALLINE STRUCTURE
TEM/SAED - Single nanoparticle can be analyzed - similar to XRD but unique in that area as small as few nanometers in size can be examined
XRD
Raman- has been used to characterize iron colloids Each crystalline form of iron oxide and iron oxyhydroxide exhibits a characteristic
Mössbauer spectroscopy spectra provide quantitative information on "hyperfine interactions" which are small energies from the interactions between the nucleus and its neighboring electrons
3.IRON ENVIRONMENT
Mössbauer spectroscopy- give information valence and spin state of iron and type of coordination of iiron (octahedral ? )
EPR
spectra of one iron colloid sample measured at different
temperatures can provide information regarding iron core
environment and paramagnetic iron structure
Raman,
UV/Vis - coordination state between iron atoms and ligands
1.CORE SIZE AND MORPHOLOGY
TEM ( measure the diameter and morphology of the iron core of iron colloid products) - carbohydrate shell usually cannot be detected
AFM ( provides a three-dimensional visualization of iron carbohydrate colloid complex )
Mössbauer spectroscopy-characterize iron colloid
XRD- peak broadening and peak symmetry iron core crystallite size and shape can be measured by XRD
4.MAGNETIC PROPERTIES
Fe (III) to Fe (II) reduction potential-Polography
Fe (II) content -Cerimetric Titration (Fe(II)- Fe(III)
- Ferrozine Assay ( Fe(II)
Mössbauer spectroscopy-Çekirdek ile çevresindeki manyetik alan arasındaki etkileşimleri temsil eder
VSM-SQUID ( General magnetic properties )
ESR ( Electron Spin Resonance)
Paramanyetik maddelerin manyetik davranışlarını karakterize eder
Elektronların çevresindeki çekirdeklerle etkileşimleri ölçülebilir
Whole particle
Equivalence in stoichiometric ratios of iron , free and bound carbohydrate and other relevant components
AAS ICP
Molecular weight distribution
AUC or GPC
Low molecular weight iron species -Free IRON
Dialysis or Ultrafiltration
Physically separated from iron colloids by dialysis or ultrafiltration 12-14 kDa cutoff membrane over a period of up to 270 min is recommended for the measurement of low mo. we iron
Labile Iron
Bleomycin assays
Chelation assays
Bleomycin Assays DNA damage is then quantified by the formation of MDA from the deoxyribose moiety of the DNA using the TBA test -UV-532 nm
incubated with DNA
Chelation Assays
Ferrozine Not only low molecular weight iron but olsa labile iron and transferrin -bound iron in serum . Iron colloid sample incubated in human plasma . ( Detergent, thiourea , citric acid , sodium ascorbate )
lower pH to free Fe(III) from transferrin and ascorbic acid reduces Fe(III) to Fe(II) . UV-562
Particle Size Distribution
DLS or AFM
DLS can be used to determine hydrodynamic size of iron colloids
CARBOHYDRATE SHELL
Carbohydrate composition and carbohydrate -iron core interactions
FTIR( interactions between carbohydrate shell and the iron core )
TGA ( use the characterize carbohydrate shell of iron colloids )
Surface charge
Zeta Potential
Potentiometric Titration
Characterization of
polysaccharides
NMR -Evans effect inappropriate to use NMR to characterize carbohydrate bonded to ferric iron core ( isolated to carbohydrates bonded to iron core )
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