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" A novel and efficient nicotine vaccine using nano-lipoplex as a…
" A novel and efficient nicotine vaccine
using nano-lipoplex as a delivery vehicle" - Dr. Chenming Zhang
Challenges Being Solved
nicotine addiction
stimulate mesolimbic dopamine neurons
induce rewarding behavior :confetti_ball:
short-term solutions :clock3:
nicotine replacement therapy
bupropion
varenicline
anti-nicotine vaccines
Connections to Global Issues
tobacco smoking
killed 6 million :skull_and_crossbones:
economic damage
hard to quit
opioid addiction
previously legal/perscribed
current American health crisis
Links to Systems
immune system :<3:
antibodies to clear substances
virus
bacteria
proteins
nicotine inhibits function
world population :silhouettes:
overall increase in health :hospital:
decreased tobacco expenses :moneybag:
Strategies Used
cationic liposomes
synthesis of nicotine conjugates
EDC
size exclusion chromatography
pH balancing
quantification
unreacted groups before conjugation
unreacted groups after conjugation
purification by dialysis
DLS analysis
testing on mice :mouse2:
characterizing physiochemical properties
TEM analysis
zetasizer
flow cytometer
Potential Solutions
new delivery system :email:
increased antigen presentation
increased hapten loading capacity
liloplex nanoparticle
nicotine linked to BSA
converted to cationic liposomes
control Nic on nanoparticle
adjusting Nic:BSA
adjusting Nic-BSA:maleimide
Future/New Challenges
BSA not clinically approved
need medically acceptable
need immunologically effective
increase nicotine epitope density :chart_with_upwards_trend:
test antibodies
only titers were evaluated
need specificity to nicotine
modify liloplex
enhance antibody response
target delivery system