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Determination of hormones in bacterial liquid by high resolution mass…
Determination of hormones in bacterial liquid by high resolution mass spectrometry
1. Phytohormone Extraction
Rhizobium TRM95796 & TRM96018
Micromonospora
TRM95362
7. Collision Energy
Low Energy: 6.0 eV.
High Energy Ramp: 20-45 eV.
5. Gas Flow Rates: Cone Gas: 50 L/h.
Desolvation Gas: 1000 L/h.
4. Mass Spectrometry Settings:
Analyzer Mode: Sensitivity.
Capillary Voltage: 2.5 kV.
Source Temperature: 120°C.
Desolvation Temperature: 500°C.
2. Identification by Mass Spectrometry: Ultra-High-Performance Liquid Chromatography-Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QToF-MS).
Mobile Phases: A: 0.1% Formic Acid Water: B: Methanol.
6.Scan Settings:
Low Mass: 50 m/z.
High Mass: 2000 m/z.
Scan Time: 0.2 seconds.
3. Chromatographic Conditions: Gradient:
0-12 min: 95% A, 5% B: Flow Rate: 0.3 mL/min.**