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entosis, inhibit ROCK, Rhabdomyosarcoma, RD (035_Rhabdomyosarcoma-Derived…

- - - - Entosis could occur when the cells are detached
        
        How can cell be detached?
        
        Use poly-HEMA coating plate
        
        How to make poly-HEMA coating plate
        
        004_Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon
        
        005_Enrichment of Melanoma Stem-Like Cells via Sphere Assays
        
        006_Rapid Generation of Single-Tumor Spheroids for High-Throughput Cell Function and Toxicity Analysis
        
        007_The relevance of using 3D cell cultures, in addition to 2D monolayer cultures, when evaluating breast cancer drug sensitivity and resistance
        
        Finally, we bought ultra low attachment plate
      - Cell engulfment requires actin, myosinⅡ, Rho, and ROCK activity
        
        How to block them
        
        actin inhibiter
        
        latruncluin B(Lat B)
        
        myosin inhibiter
        
        Blebbistain
        
        ROCK inhibiter
        
        Y27632
        
        051_Calciumsensitization of smooth muscle mediated by a Rho-associated
        protein kinase in hypertension
        
        H1152
        
        Rho, ROCK activity in sarcoma
        
        023_Differential roles of Rho-kinase and myosin light chain kinase in regulating shape, adhesion, and migration of HT1080 fibrosarcoma cells
        
        Pharmacological inhibition of Rho-kinase by Y27632 or inhibition of MLCK by W-7 or ML-7 resulted in significant attenuation of
        
        MLCK inhibitor
        
        ML-7
        
        3 more items...
        
        W-7
      - Entosis Results in Lysosomal Cell Death
        
        034_Cell-in-Cell Death is not Restricted by Caspase-3 Deficiency in MCF-7 Cells
        
        emperitosis
        
        caspase-dependent cell-in-cell apoptotic death
        
        entosis
        
        nonapoptotic lysosome dependent cell-in-cell death
        
        Cell Culture
        
        A431
        
        MCF7
        
        Partial deletion of the CASP-3 gene in MCF-7 cells
      - Cell Internalization Occurs Independent of Apoptotic Processes
        
        The addition of PS-liposomes, which can inhibit phagocytosis by overwhelming the PSR, also had no effect on cell internalization
        
        033_EXPOSURE OF PHOSPHATIDYLSERINE ON THE SURFACE OF APOPTOTIC LYMPHOCYTES TRIGGERS SPECIFIC RECOGNITION AND REMOVAL BY MACROPHAGES
      - Entotic cell death
        
        050_Role and dynamics of vacuolar pH during cell-in-cell mediated death
        
        inner cells could be killed non-autonomously by the outer cells that are lysosome-dependent and preceded by a transient pre-death LC3 lipidation onto the entotic vacuole
  - - - Cells which have no cadherin never occur entosis
      - entosis is driven by differences in polarized ROCK-regulated cortical actomyosin
    - - overexpression of Lgl1/2 or depletion of endogenous Par3 leads to enhanced myosin Ⅱ activation
        
        What is Par3 and Lgl
        
        016_The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
    - - PCDH7 is a Negative Regulator of Homotypic Cell-in-Cell formation
      - PCDH7 Enhances Actomyosin Contraction and Cell Stiffness
        
        PCDH7 may inhibit hoCIC formation by upregulating pMLC2, specifically at cell-cell contact where PCDH7 is highly enriched
        
        Is there pMLC2 in osteocarcinoma?
        
        019_3-hydoroxyflavone inhibits human osteosarcoma U2OS and 143B cells metastasis by affecting EMT and repressing u-PA/MMP-2 via FAL-Src to MEK/ERK and RhoA/MLC2 pathways and reduces 143B tumor growth in vivo
    - - G-protein-coupled LPA receptor 2 as a signal transducer specifically required for the actively invading cell during entosis.
      - G12/13 and PDZ-RhoGEF are required for entosic invasion, which is driven by blebbing and a uropod-like actin structure at the rear of the invading cell
      - Cells activated diaphanous-related formin1 (mDia1) with higher RhoA activity, thereby producing biophysical forces that drive penetration
    - - α-catenin lack cell might not occur entosis
      - α-catenin induce f-actin moving to membrane
        
        045_Structure and Biochemistry of Cadherins
        
        a-catenin has the capacity to affect f-actin movement
        
        Let's detect whether RD has a-catenin or not
    - - Reduced CDKN2A expression promotes CIC formation
      - CDKN2A reduces the amount of F actin
      - CIC formation negatively correlates with p16INK4a expression in breast cancers
  - - - Professor doesn't like this theory
  - - - CICs are important biological players
      - CICs mediate inner cell death
      - Stepwise formation of entotic CICs
      - Contractile actomyosin
      - Adherens junction
      - Mechanical ring
        
        021_Mechanical Ring Interfaces between adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation
        
        The ring-like structure is a multi-molecular complex consisting of adhesive and cytoskeletal proteins, in which the mechanical sensor vinculin is highly enriched.
        
        MR actively intracts with CA and the AJ to help establish and maintain polarized actomyosin that drives cell intermalization
      - Regulatory factors
        
        022_Cholesterol inhibits entotic cell-in-cell formation and actomyosin contraction
  - - - 001_Emperipolesis, entosis and cell cannibalism: Demystifying the cloud
- - - - E-cadherin
    - - CD44
        
        +
        
        Osteosarcoma
        
        MG64
        
        027_CD44 Enhances Tumor Formation and Lung Metastasis in Experimental Osteosarcoma and Is an Additional Predictor for Poor Patient Outcome
        
        028_Targeting CD44 by CRISPR-Cas9 in Multi-Drug Resistant Osteosarcoma Cells
        
        FIbrosarcoma
        
        HT1080
        
        increased expression of CD44 was correlated with worse outcomes in soft tissue sarcomas
        
        025_CD44 In Sarcomas: A comprehensive Review and Future Perspectives
        
        ±
        
        We bought
        
        22/10/03 I tried IHC on RD
        
        success
      - N-cadherin
        
        ＋
        
        Rhabdomyosarcoma
        
        041_N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling
        
        Osteosarcoma
        
        fibrosarcoma
        
        tissue cannot be identified
        
        ー
        
        Osteosarcoma
        
        041_N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling
        
        Osteoblast has N-cadherin but when it turn to malignant, N-cadherin expression is decrease
        
        042_Anomalous Cadherin Expression in Osteosarcoma
    - - 040_The cadherins: cell-cell adhesion molecules controlling animal
        morphogenesis