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Make 4 tubes of 2-fold serial dilutions with a stock BSA solution (A). As…

- - - - Take absorbance readings from the P, N, and B cuvettes at 400 nm and at every 25 nm increase until reaching 700 nm
        
        Find the wavelength best suited for measuring protein concentration (e.g. high absorbance for P but low absorbance for N)
        
        Record the absorbance values for BSA diluted cuvettes using a spectrophotometer at the appropriate wavelength (595 nm)
        
        End: Use the γ-globulin standard curve to determine the protein concentration of the unknown BSA stock solution
        
        Take the absorbance value of each known γ-globulin concentration cuvettes at the appropriate wavelength (595 nm)
        
        Construct a standard curve graph of absorbance vs γ-globulin concentration
- - - - Make cuvettes for the known γ-globulin concentrations (0.00 mg/mL, 0.28 mg/mL, 0.56 mg/mL, 0.84 mg/mL, 1.12 mg/mL, and 1.4 mg/mL)
        
        Add 2.5 mL of Bradford reagent for each cuvette