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Introduction to Chromatography - Coggle Diagram
Introduction to Chromatography
Stationary phase = remains fixed
Mobile phase = has mobility
Retention Time = Time that a solutes spends in column
Elution Techniques
Isocratic Elution = Steady changes of the Mobile Phase composition
Gradient elution = Mobile phase remains constant
Resolution
Types of chromatography
Based on arrangement of stationary phase
Planar Chromatography ( TLC, Paper)
Column Chromatography
Preparative Chromatography
Based on Stationary Phase
Solid/ Adsorption Chromatography (TLC)
Liquid/ Absorption/Partition Chromatography (Paper)
Others
Size exclusion Chromatography
Affinity Chromatography
Ion Exchange Chromatography
Normal Phase(TLC)
Reverse Phase( HPLC)
Fronting and Tailing
Asymmetric factor, Capacity factor, Selectivity factor
Types of Standard
Reference Standard
Working standard
Internal Standard
Development Techniques
Ascending
Descending
Radial
2-Dimensional(2-D)
Detection Techniques
Physical (UV chamber) = recovery is possible
Chemical= use of chemical reagents,destroys the nature of analyte