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Chromatography (Separation based on colour) - Coggle Diagram
Chromatography
(Separation based on colour)
Important phases of chromatography
Stationary phase
Polarity of an analyte
Mobile phase
Normal phase (Ex-:TLC)
Stationary phase-:polar
Nature of an analyte-:Non polar
Mobile Phase-:Non polar
Elute First-:Non polar
Reverse phase (Ex-:HPLC)
Stationary phase-: Non polar
Nature of an analyte-:polar
Mobile Phase-: polar
Elute First-: polar
Based on detection
Physical
(UV chamber)
Chemical
(Reagents)
Development technique of paper chromatography
Descending
(mobile phase moves downward through the stationary phase)
Radial
(principle of this method is based on migration of the spot from the centre of the paper to the periphery)
Ascending
(mobile phase moves upward through the stationary phase)
Two dimensional
(which enhances the separation of a mixture into individual components)
Elution
Isocratic-:
(one mobile phase)
Gradient-:
(more than one mobile phase)