UV visible spectroscopy
Electronic transition
Sigma to Sigma - eg:- alkanes
n to sigma eg: -OH
Pi to Pi eg: saturated ketones
n to Pi eg: aliphatic ketone
Chromophore
Independent
Dependent
Spectral shift
Bathochromic shift
Hypsochromic shift
Hyperchromic shift
Hypochromic shift
Auxochrome
Molecule which intensifies
the absorption when attached
to a chromophore
eg: -OH, -NH2
Instrumentation
Wavelength selector
Cells or cuvette
Radiation source
Detector
Uv
Visible
Filter
Monochromator
Slit
Photomultiplier tube
Linear diode array
Linear photo diode array
Beer lamberts law
A=abc
where, A= Absorbance
a=molar absorptivity
b= path length
c=concentration