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HPLC - Coggle Diagram
HPLC
Instrumentation
Solvent Reservoir and Degassing System
Pumping System
Sample Injection system
Columns
Guard Columns
Thermostats
Detectors
Bulk Property Detector
Solute property Detector
Multipurpose Detector
Electrochemical Detector
Stripcart Recorder
Data handling system
Principle
Partition
Ion Exchange
Adsortion
Exclusion
Application
Stability Study
Design of Dosage form
Bioassay
Assay of Cephalosporins
bioanalytical
Advantages
Better Resolution
Less time
More accurate and Precision and sensitivity
Introduction :
High performance liquid Chromatography (HPLC)
High Rapid process with HIGH RESOLUTION and High SPEED
Theory
Mobile Phase
Polar
non- polar
Stationary Phase
HPLC COLUMN
Component separated according to their Interactions with column pack