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Chromatography - Coggle Diagram
Chromatography
Types of
chromatography
Based on
Stationary
Phase
Solid/
Adsorption
Chromato-
graphy
(TLC)
Liquid/
Absorption/
Partition
Chromato-
graphy
(Paper)
Based on
arrangement of
stationary
phase
Column
Chromatography
Preparative
Chromatography
Planar
Chromatography
( TLC, Paper)
Normal
Phase(TLC)
Reverse
Phase( HPLC)
Others
Ion Exchange
Chromatography
Affinity
Chromatography
Size exclusion
Chromatography
Factors
Asymmetric
factor
Tailing factor
Capacity factor
Fronting & tailing factor
Development
Techniques
Ascending
Descending
2D
Radial
Types of
Standard
Reference
Standard
Working Standard
Internal Standard
Elution
Techniques
Isocratic Elution =
Steady changes of
the Mobile Phase
composition
Gradient elution =
Mobile phase
remains constant
Detection
Techniques
Physical (UV
chamber) =
recovery is
possible
Chemical= use
of chemical
reagents,destro
ys the nature of
analyte
Stationary phase
= remains fixed
Mobile phase =
has mobility
Retention Time
= Time that a
solutes spends
in column
Resolution