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Chromatography - Coggle Diagram
Chromatography
Related terms
Mobile phase :
Moves in specific direction
Stationary phase
Bonded on supporting particles
Analyte
Components that get separated
Effluent
Mobile phase coming out of column
Types
Solid
(TLC)
-Principle:- Adsorption
-Reverse phase possible
Liquid
(Paper chromatography)
Principle:- Absorption
Reverse phase is not possible
Gas
(Gas chromatography)
Gas should be non reactive
Development technique
Ascending
Descending
Radial
Arrangement based classification
Planar chromatography
Column chromatography
Preparative chromatography
Detector
Physical detector
Ex:- UV -visible spectroscopy
Chemical detector
Ex:-chemical agent
Definition
It is combination of laboratory techniques that are used for separating the mixture of components
Normal phase chromatography
Nonpolar mobile phase
Polar stationary phase
Non polar analyte
Ex :- TLC
Reverse phase chromatography
Polar mobile phase
Non polar stationary phase
Polar analyte
Ex:- HPLC
Reference standard
Highest purity
Working standard
Not much purity
Internal standard
Structurally similar
Tailing
Good interaction of analyte with stationary phase
Fronting
Loading high amount of sample
Resolution
Separation of two peak
Signal of noise ratio
Less than 10% peak area is noise area
Capacity factor
Maximum ability to perform separation
Selectivity factor
Ability to separate specific analyte