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ESCHERICHIA COLI - Coggle Diagram
ESCHERICHIA COLI
MORPHOLOGY AND CULTURAL CHARACTERISTICS
gram negative bacilli
motile
non-sporing
non-sporing
ON BLOOD AGAR (hemolysis)
grey moist colony
ON MACCONKEY AGAR
lactose fermenting
**BIOCHEMICAL REACTIONS
IMViC = ++- -
INDOLE TEST
positive
METHYL RED TEST
positive
VOGES-PROSKAUER TEST
negative
CITRATE TEST
negative
INFORMATION ABOUT E.COLI
member of normal intestinal flora
opportunistic pathogens
may reach blood stream and cause sepsis
also can reach to other organ, e.g UTI
VIRULENCE FACTORS
surface antigen
lipopolysaccharide O antigen:
endotoxic activity
anti-phagocytic in nature
Fimbriae antigens 'F'
initial attachmentl & colonisation
K antigens (capsule)
Protect against phagocytosis
Toxins
Enterotoxins
all of them responsible for diarrhoea
Heat labile toxin (HLT)
Heat stable toxin (ST)
Verotoxin
Haemolysin
No role in pathogenesis
